Our work


Our works are below. * means Corresponding author.

2018Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles.Itoh, K., Akimoto, Y., Kondo. S., Ichimiya, T., Aoki, K., Tiemeyer. M., Nishihara. S.*Dev Biol. 436, 108-124 (2018).
2018O-GlcNAc on PKCζ inhibits the FGF4-PKCζ-MEK-ERK1/2 pathway via inhibition of PKCζ phosphorylation in mouse embryonic stem cells.Miura, T., Kume, M., Kawamura, T., Yamamoto, K., Hamakubo, T., Nishihara, S.*Stem Cell Reports, 10, 1–15 (2018).
2017Strong radioprotective FGF1 signaling down-regulates proliferative and metastatic capabilities of the angiosarcoma cell line, ISOS-1, through the dual inhibition of EGFR and VEGFR pathways.Miura, T., Fujita, M., Kawano, M., Imadome, K., Yasuda,T., Nishihara, S., Imamura, T., Masuzawa, M., Imai, T., Nakayama, F.Clin Transl Radiat Oncol. 7, 83-90 (2017).
2017Short stop mediates axonal compartmentalization of mucin-type core 1 glycans.Kinoshita, T., Sato, C., Fuwa, T.J., Nishihara, S.*Sci Rep., 7, 41455 (2017).
2016O-GlcNAc is required for the survival of primed pluripotent stem cells and their reversion to the naïve state.Miura, T., Nishihara, S.*BBRC, 480, 655-661 (2016).
2016Mucin-type core 1 glycans regulate the localization of neuromuscular junctions and establishment of muscle cell architecture in Drosophila.Itoh, K., Akimoto, Y., Fuwa, T.J., Sato, C., Komatsu, A., Nishihara, S.*Dev Biol., 412, 114-127 (2016).
2016Atmospheric-pressure plasma-irradiation inhibits mouse embryonic stem cell differentiation to mesoderm and endoderm but promotes ectoderm differentiation.Miura, T., Hamaguchi, S., Nishihara, S.*J. Phys. D: Appl. Phys., 49, 165401-1 - 165401-12 (2016).
2015Preparation of a polyclonal antibody that recognizes a unique galactoseβ1-4fucose disaccharide epitope.Takeuchi, T., Nishiyama, K., Saito, S., Tamura, M., Fuwa, T.J., Nishihara, S., Takahashi, H., Natsugari, H,. Arata, Y., Kasai, K.Carbohydr Res. 412, 50-55 (2015).
2015Identification of β1,3-galactosyltransferases responsible for biosynthesis of insect complex-type N-glycans containing a T-antigen unit in the honeybee.Ichimiya, T., Maeda, M., Sakamura, S., Kanazawa, M., Nishihara, S.*, Kimura, Y.Glycoconjugate J., 32, 141-151 (2015).
2015Phenotype-based clustering of glycosylation-related genes by RNAimediated gene silencing.Yamamoto-Hino, M., Yoshida, H., Ichimiya, T., Sakamura, S., Maeda, M., Kimura, Y., Sasaki, N., Aoki-Kinoshita, K.F., Kinoshita-Toyoda, A., Toyoda, H., Ueda, R., Nishihara, S.*, Goto, S.Genes to Cells, 20, 521-542 (2015).
2015Reduction of T antigen causes loss of hematopoietic progenitors in Drosophila through the inhibition of filopodial extensions from the hematopoietic niche.Fuwa, T.J., Kinoshita, T., Nishida, H., Nishihara, S.*Dev Biol., 401, 206-219 (2015).
2014A chicken influenza virus recognizes fucosylated α2,3 sialoglycan receptors on the epithelial cells lining upper respiratory tracts of chickens.Hiono, T., Okamatsu, M., Nishihara, S., Takase-Yoden, S., Sakoda, Y., Kida, H.Virology, 456–457, 131–138 (2014).
2014Proliferation assay of mouse embryonic stem (ES) cells exposed to atmosphericpressure plasmas at room temperature.Miura, T., Ando, A., Hirano, K., Ogura, C., Kanazawa, T., Ikeguchi, M., Seki, A., Nishihara, S., Hamaguchi, S.J. Phys. D: Appl. Phys. 47(44) 445402-1 - 445402-12 (2014).
2014Electron microscopy of primary cell cultures in solution and correlative optical microscopy using ASEM.Hirano, K., Kinoshita, T., Uemura, T., Motohashi, H., Watanabe, Y., Ebihara, T., Nishiyama, H., Sato, M., Suga, M., Maruyama, Y., Tsuji, N.M., Yamamoto, M., Nishihara, S.* , Sato, C.Ultramicroscopy,143, 52-66 (2014).
2014Immuno-electron microscopy of primary cell cultures from genetically modified animals in liquid by atmospheric scanning electron microscopy.Kinoshita, T., Mori, Y., Hirano, K., Sugimoto, S., Okuda, K., Matsumoto, S., Namiki, T., Ebihara, T., Kawata, M., Nishiyama, H., Sato, M., Suga, M., Higashiyama, K., Sonomoto, K., Mizunoe, Y., Nishihara, S.*, Sato, C. Microsc Microanal, 20, 470-484 (2014).
2014Frequent glycan structure mining of influenza virus data revealed a sulfated glycan motif that increased viral infection.Ichimiya, T., Nishihara, S., Takase-Yoden, S., Kida, H., Aoki- Kinoshita, K.F.Bioinformatics, 30, 706-711(2014).
2013The transition of mouse pluripotent stem cells from the naïve to the primed state requires Fas signaling through 3-O sulfated heparan sulfate structures recognized by the HS4C3 antibody.Hirano, K., Van Kuppevelt, T.H., Nishihara, S.*BBRC, 430, 1175–1181 (2013).
2013Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines.Nakayama, F., Umeda, S., Ichimiya, T., Kamiyama, S., Hazawa, M., Yasuda, T., Nishihara, S., Imai, T.FEBS Lett., 587, 231-783 (2013).
20123-O-sulfated heparan sulfate recognized by the antibody HS4C3 contribute to the differentiation of mouse embryonic stem cells via Fas signaling.Hirano, K., Sasaki, N., Ichimiya, T., Miura , T., Van Kuppevelt ,T.H., Nishihara, S.*PLoS One, 7, e43440 (2012).
2012O-sulfate groups of heparin are critical for inhibition of ecotropic murine leukemia virus infection by heparin.Seki, Y., Mizukura, M., Ichimiya, T., Suda, Y., Nishihara, S., Masuda, M., Takase-Yoden, S.Virology, 424, 56-66 (2012).
2011LacdiNAc (GalNAcβ1-4GlcNAc) contributes to self-renewal of mouse embryonic stem cells by regulating LIF/STAT3 signaling.Sasaki, N., Shinomi, M., Hirano, K., Ui-Tei, K., Nishihara, S.*Stem Cells, 29, 641-650 (2011).
2011Expression and role of 3'-phosphoadenosine 5'-phosphosulfate transporters in human colorectal carcinoma.Kamiyama, S., Ichimiya, T., Ikehara, Y., Takase, T., Fujimoto, I., Suda, T., Nakamori, S., Nakamura, M., Nakayama, F., Irimura, T., Nakanishi, H., Watanabe, M., Narimatsu, H., Nishihara, S.*Glycobiology, 21, 235-246 (2011).
2010Identification of genes required for neural-specific glycosylation using functional genomics.Yamamoto-Hino, M., Kanie, Y., Awano, W., Aoki-Kinoshita, K.F., Yano, H., Nishihara, S., Okano, H., Ueda, R., Kanie, O., Goto, S.PLoS Genet., 6, e1001254 (2010).
2010Chemical inhibition of sulfation accelerates neural differentiation of mouse embryonic stem cells and human induced pluripotent stem cells.Sasaki, N., Hirano, T., Kobayashi, K., Toyoda, M., Miyakawa, Y., Okita, H., Kiyokawa, N., Akutsu, H., Umezawa, A., Nishihara, S.*BBRC, 401, 480-486 (2010).
2010Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome.Ueyama, M., Akimoto, Y., Ichimiya, T., Ueda, R., Kawakami, H., Aigaki, T., Nishihara, S.*PLoS One, 5, e11557 (2010).
2010Two Golgi-resident 3' -phosphoadenosine 5' -phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.Dejima, K., Murata, D., Mizuguchi, S., Nomura, K.H., Izumikawa, T., Kitagawa, H., Gengyo-Ando, K., Yoshina, S., Ichimiya, T., Nishihara, S., Mitani, S., Nomura, K.J Biol Chem., 285, 24717-24728 (2010).
2010Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen function redundantly in the O-fucosylation of Notch in Drosophila.Ishikawa, H.O., Ayukawa, T., Nakayama, M., Higashi, S., Kamiyama, S., Nishihara, S., Aoki, K., Ishida, N., Sanai, Y., Matsuno, K.J Biol Chem., 285, 4122-4129 (2010).
2009The 3'-phosphoadenosine 5'-phosphosulfate transporters, PAPST1 and 2, contribute to the maintenance and differentiation of mouse embryonic stem cells.Sasaki, N., Hirano, T., Ichimiya, T., Wakao, M., Hirano, K., Kinoshita-Toyoda, A., Toyoda, H., Suda, Y., Nishihara, S.*PLoS One, 4, e8262 (2009).
2009The ortholog of human solute carrier family 35 member B1 (UDP-galactose transporter-related protein 1) is involved in maintenance of ER homeostasis and essential for larval development in Caenorhabditis elegans.Dejima, K., Murata, D., Mizuguchi, S., Nomura, K.H. Gengyo-Ando, K., Mitani, S., Kamiyama, S., Nishihara, S., Nomura, K.FASEB J., 23, 2215-2225 (2009).
2009 ER/Golgi nucleotide sugar transporters contribute to the cellular release of UDP-sugar signaling molecules.Sesma. J.I., Esther, C.R. Jr, Kreda, S.M., Jones, L., O'Neal, W., Nishihara, S., Nicholas, R.A., Lazarowski, E.R.J. Biol. Chem., 284, 12572-12583 (2009).
2009Insight into the regulation of glycan synthesis in Drosophila chaoptin based on mass spectrometry.Kanie, Y., Yamamoto-Hino, M., Karino, Y., Yokozawa, H., Nishihara, S., Ueda, R., Goto, S., Kanie, O.PLoS ONE, 4, e5434 (2009).
2008Identification of the Drosophila core 1 β1,3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes.Yoshida, H., Fuwa, T.J., Arima, M., Hamamoto, H., Sasaki, N., Ichimiya, T., Osawa, K., Ueda, R., Nishihara, S.*Glycobiology, 18, 1094-1104 (2008).
2008Sequential glycosyltransfer reactions on a microfluidic device: Synthesis of a glycosaminoglycan linkage region tetrasaccharide.Ono, Y., Kitajima, M., Daikoku, S., Shiroya, T., Nishihara, S., Kanie, Y., Suzuki, K., Goto, S., Kanie, O.Lab. on a Chip, 8, 2168-2173 (2008).
2008Functional analysis of proteoglycan galactosyltransferase II RNAi mutant flies.Ueyama, M., Takemae, H., Ohmae, Y., Yoshida, H., Toyoda, H., Ueda, R., Nishihara, S.*J. Biol. Chem., 283, 6076-6084 (2008).
2008Heparan sulfate regulates self-renewal and pluripotency of embryonic stem cells.Sasaki, N., Okishio, K., Ui-Tei, K., Saigo, K., Kinoshita-Toyoda, A., Toyoda, H., Nishimura, T., Suda, Y., Hayasaka, M., Hanaoka, K., Hitoshi, S., Ikenaka, K., Nishihara, S.*J. Biol. Chem., 283, 3594-3606 (2008).
2007Drosophila β1,4-N-acetylgalactosaminyltransferase-A synthesizes the LacdiNAc structures on several glycoproteins and glycosphingolipids.Biochem Biophys Res Commun, 354, 522-527 (2007).Sasaki, N., Yoshida, H., Fuwa, T.J., Kinoshita-Toyoda, A., Toyoda, H., Hirabayashi, Y., Ishida, H., Ueda, R., Nishihara, S.*
2007Mice lacking α1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors.Kudo, T., Fujii, T., Ikegami, S., Inokuchi, K., Takayama,Y., Ikehara, Y., Nishihara, S., Togayachi, A., Takahashi, S., Tachibana, K., Yuasa, S., Narimatsu, H.Glycobiology, 17, 1-9 (2007).
2006Involvement of Drosophila Sir2-like genes in the regulation of life span.Kusama, S., Ueda, R., Suda, T., Nishihara, S., Etsuko, T.Genes & Genetic Systems, 81, 341-348 (2006).
2006Identification and characterization of a novel Drosophila 3'-phosphoadenosine 5'-phosphosulfate transporter.Goda, E., Kamiyama, S., Uno, T., Yoshida, H., Ueyama, M., Kinoshita-Toyoda , A., Toyoda, H., Ueda, R., Nishihara, S.*J Biol Chem, 281, 28508-28517 (2006).
2006Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2.Kamiyama, S., Sasaki, N., Goda, E., Ui-Tei, K., Saigo, K., Narimatsu, H., Jigami, Y., Kannagi, R., Irimura, T., Nishihara, S.*J Biol Chem, 281, 10945-10953 (2006).
2005β4GalT-II is a key regulator of glycosylation of the proteins involved in neuronal development.Sasaki, N., Manya, H., Okubo, R., Kobayashi, K., Ishidad,H., Toda, T., Endo, T., Nishihara, S.*Biochem Biophys Res Commun, 333, 131-137 (2005).
2004The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity.Ichimiya, T., Manya, H., Ohmae, Y., Yoshida, H., Takahashi, K., Ueda, R., Endo, T., Nishihara, S.*:J Biol Chem, 279, 42638-42647 (2004).
2004Drosophila glucosylceramide synthase: A negative regulator of cell death mediated by proapoptotic factors.Kohyama-Koganeya, A., Sasamura, T., Oshima, E., Suzuki, E., Nishihara, S., Ueda, R., Hirabayashi Y.J Biol Chem, 279, 35995-6002 (2004).
2004Molecular cloning and characterization of a human multi-substrate specific nucleotide-sugar transporter homologous to Drosophila fringe connection.Suda,T., Kamiyama, S., Suzuki. M., Kikuchi N., Nakayama., Narimatsu H., Jigami , Y., Aoki, T., Nishihara, S.*:J Biol Chem, 279, 26469-26474 (2004).
2004Normal embryonic and germ cell development in mice lacking α1,3-fucosyltransferase IX (Fut9) which show disappearance of stage-specific embryonic antigen 1.Kudo, T., Kaneko, M., Iwasaki, H., Togayachi, A., Nishihara, S., Abe, K., Narimatsu. H.Mol Cell Biol, 24, 4221-4228(2004).
2004Design and synthesis of peptide mimetics of GDP-Fucose: Targeting Inhibitors of fucosyltransferases.Tanaka, T, Tsuda, C., Miura, T., Inazu, T., Tsuji, S., Nishihara, S., Hisamatsu, M., Kajimoto, T.Synlett, 2004, 243-246 (2004).
2003Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter.Kamiyama, S., Suda, T., Ueda, R., Suzuki, M., Okubo, R., Kikuchi, N., Chiba, Y., Goto, S., Toyoda, H., Saigo, K., Watanabe, M., Narimatsu, H., Jigami, Y., Nishihara, S.*J Biol Chem, 278, 25958-25963 (2003).
2003Proteoglycan UDP-galactose: β-xylose β1,4galactosyltransferase I is essential for viability in Drosophila MelanogasterTakemae, H., Ueda, R., Ohkubo, R., Nakato, H., Izumi, S., Saigo, K., Nishihara, S.*J Biol Chem, 278, 15571-15578 (2003).